8/28/2023 0 Comments Kcn serial macroCultured cells that were treated with cytokines, or that were cocultivated with cytokine-activated macrophages, regained EPR active complexes. Tumors grown in vivo contained EPR signals while those grown in culture without continuing cytokine stimulation lost the signals after a few days. Signal amplitudes were attenuated in mice treated with N omega-mono-methyl-L-arginine (MLA), an inhibitor of nitric oxide synthase. Hemoglobin assays showed that the heme-nitrosyl signals were not derived from contaminating hemoglobin. Three different EPR active iron-nitrosyl species were observed, an Fe(RS)2(NO)2 signal and two differentiable heme-nitrosyl complexes. We observed that syngeneic murine tumors exhibited EPR signals related to iron-nitrosyl complex formation. The cell-mediated immune response to syngeneic tumors activates the cytokine-inducible nitric oxide synthase.
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